홀리바질 (적응원)

Effects of multi-herb and ashwagandha root formulas on stress modulation: a randomized, double-blind, placebo-controlled clinical study.

1. Trials. 2026 Feb 9;27(1):205. doi: 10.1186/s13063-026-09495-9. Effects of multi-herb and ashwagandha root formulas on stress modulation: a randomized, double-blind, placebo-controlled clinical study. McKinney E(1), Stewart J(1), Kewalramani R(2), Singh S(3). Author information: (1)R&D Departm

Chronic supplementation of a multi-ingredient herbal supplement increases speed of cognitive task performance alongside changes in the urinary metabolism of dopamine and the gut microbiome in cognitively intact older adults experiencing subjective memory decline: a randomized, placebo controlled, parallel groups investigation.

1. Front Nutr. 2023 Oct 10;10:1257516. doi: 10.3389/fnut.2023.1257516. eCollection 2023. Chronic supplementation of a multi-ingredient herbal supplement increases speed of cognitive task performance alongside changes in the urinary metabolism of dopamine and the gut microbiome in cognitively intact older adults experiencing subjective memory decline: a randomized, placebo controlled, parallel groups investigation. Wightman E(1)(2), Khan J(1), Smith E(1), Rolfe V(3), Smith D(1)(2)(4), Young G(1)(2)(4), Cheung W(1), Kennedy D(1)(2). Author information: (1)Northumbria University, Newcastle upon Tyne, United Kingdom. (2)Nutrition Trials at Northumbria (NUTRAN), Newcastle upon Tyne, United Kingdom. (3)Pukka Herbs Ltd.,, The Chocolate Factory, Keynsham, Bristol, United Kingdom. (4)NU-OMICS, Northumbria University, Newcastle upon Tyne, United Kingdom. BACKGROUND: The effects of herbs on brain function are often investigated in isolation, yet herbal preparations are often complex combinations of phytochemicals, designed to target widespread mechanisms. OBJECTIVE: To assess the effects of chronic, 12 weeks, supplementation of a multi-ingredient herbal supplement (containing Bacopa monnieri, Gotu kola leaf, Turmeric whole powder, Reishi full spectrum, Rosemary, Cardamom, Holy Basil, Turmeric Wholistic™ extract, Green Tea & Seagreens) on cognitive function in older adults with subjective memory decline. Secondly, to investigate whether effects are underpinned by shifts in microbial composition and/or metabolism of the herbs. METHODS: Male and female participants (N = 128) aged between 55-75 years completed lab-based cognitive assessments, and provided stool and urine samples, at baseline and then following 90 days of multi-ingredient herb, or placebo, supplementation. RESULTS: Deficits in memory were observed in response to 90 days of multi-ingredient herbal supplement supplementation but the positive effects were all focused on speed of cognitive task performance, with an additional improvement in the false alarm rate on the rapid visual information processing task. These improvements coincided with an increased presence of tyrosine in the urinary metabolome and this may implicate the role of dopamine in these processing and/or motor speed increases. Finally, multi-ingredient herbal supplementation significantly reduced levels of 3 bacterial species in the gut microbiome and one of these, Sutterella, coincides with lower levels of constipation reported in the multi-ingredient herbal supplement condition. CONCLUSION: A multi-ingredient herbal supplement increases speed of cognitive task performance and increased metabolism of tyrosine suggests that this is modulated by increased dopaminergic activity. Reduced levels of Sutterella in the gut is associated with improved bowel movements of participants. Interpretation of the negative effects on memory are, however, stymied by an unequal randomization of participants into treatment groups pre- and post-COVID 19.Clinical trial registration: identifier NCT05504668. Copyright © 2023 Wightman, Khan, Smith, Rolfe, Smith, Young, Cheung and Kennedy. DOI: 10.3389/fnut.2023.1257516 PMCID: PMC10598389 PMID: 37885445 Conflict of interest statement: Author VR was employed by the company Pukka Herbs Ltd. The authors declare that this study received funding from Pukka Herbs Ltd. The funder had the following involvement in the study: preparation of the manuscript. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The potential role of the novel orodispersible film from rice polymer loaded with silkworm pupae hydrolysate and the combined extract of holy basil and ginger for the management of stroke with stress.

2. Biomaterials. 2023 Aug;299:122175. doi: 10.1016/j.biomaterials.2023.122175. Epub 2023 May 22. The potential role of the novel orodispersible film from rice polymer loaded with silkworm pupae hydrolysate and the combined extract of holy basil and ginger for the management of stroke with stress. Thongwong P(1), Wattanathorn J(2), Thukhammee W(3), Tiamkao S(4). Author information: (1)Department of Physiology and Graduate School (Neuroscience Program), Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address: putthiwat.t@gmail.com. (2)Integrative Complementary Alternative Medicine Research and Development Center in Research Institute for Human High Performance and Health Promotion, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: jinwat05@gmail.com. (3)Integrative Complementary Alternative Medicine Research and Development Center in Research Institute for Human High Performance and Health Promotion, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: meewep@gmail.com. (4)Integrative Complementary Alternative Medicine Research and Development Center in Research Institute for Human High Performance and Health Promotion, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Internal Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. Electronic address: somtia@kku.ac.th. The prevalence of stroke under stress conditions is rising and the severity of stroke is increasing. Owing to the limitation of the current therapeutic strategy, a novel effective strategy for treating this condition is needed. In this study, we explored the neuroprotective effect of an orodispersible film derived from a rice polymer loaded with silkworm pupae and the combined extract of holy basil and ginger (JP1). Male Wistar rats weighing 200-250 g were administered JP1 at the doses of 1, 10, and 100 mg/kg BW 45 min prior to an exposure to a 6-h immobilization stress for 14 days. Permanent, occlusion of the right middle cerebral artery (MCAO) was performed, and JP1 was administered continually for 21 days after MCAO. Assessments of the brain infarction volume, oxidative stress, inflammation, and apoptosis in the cerebral cortex were carried out 24 h after MCAO. Neurological severity scores were also determined for the rats every 7 days after MCAO until the end of the study period. The results clearly showed that all doses of JP1 decreased the brain infarct volume, oxidative stress, inflammation, and apoptosis and improved neurological deficits. Therefore, JP1 is a potential novel neuroprotective supplement for combating ischemic stroke under stress conditions. However, a clinical trial is essential to confirm this beneficial effect. Copyright © 2023 Elsevier Ltd. All rights reserved. DOI: 10.1016/j.biomaterials.2023.122175 PMID: 37262936 [Indexed for MEDLINE] Conflict of interest statement: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Effects of flaxseed, sardines, pearl millet, and holy basil leaves on production traits of layers and fatty acid composition of egg yolks.

3. Poult Sci. 2011 Jan;90(1):147-56. doi: 10.3382/ps.2008-00152. Effects of flaxseed, sardines, pearl millet, and holy basil leaves on production traits of layers and fatty acid composition of egg yolks. Kirubakaran A(1), Narahari D, Ezhil Valavan T, Sathish Kumar A. Author information: (1)Tamilnadu Veterinary and Animal Sciences University, Chennai, India. kirubavet@yahoo.co.in The effects of flaxseed, sardines, pearl millet, and holy basil (Oscimum sanctum) leaf meal on Single Comb White Leghorn (Forsgate strain) layer hen production traits, fatty acid composition of egg yolks, and egg quality characteristics were studied. Layer hens were allocated to 1 of 6 dietary treatments from 27 to 32 wk of age: control (treatment 1), control + 1 g of basil leaf meal (BLM) (treatment 2), control + 2 g of BLM (treatment 3), designer egg layer mash (DELM; treatment 4), DELM + 1 g of BLM (treatment 5), DELM + 2 g of BLM (treatment 6). No significant differences were found in egg production between dietary treatments, indicating that neither the designer diet nor the BLM had an adverse effect on egg production. The high energy level in the designer diet, because of the flaxseed oil and sardines, significantly reduced feed intake compared with the control diet. The egg weight was significantly higher in birds fed the designer diet compared with the egg weight in the control diet. The flaxseed oil and sardine oil, which are rich in n-3 polyunsaturated fatty acids, contributed to significantly increased n-3 polyunsaturated fatty acid levels in the egg yolks, with a proportionate reduction in the level of palmitic acid. Other fatty acids showed only minor variations. Flaxseed oil increased the level of α-linolenic acid, whereas fish oil increased the eicosapentaenoic acid and docosahexaenoic acid levels. The BLM at low levels of inclusion (1 and 2 g/kg) also contributed significantly to elevating the yolk n-3 polyunsaturated fatty acid levels, especially C18:3. Holy basil leaf meal is a good feed supplement for increasing the yolk α-linolenic acid level by reducing the yolk saturated fatty acid levels proportionately. DOI: 10.3382/ps.2008-00152 PMID: 21177454 [Indexed for MEDLINE]

Antioxidants and antiinflammatory dietary supplements for osteoarthritis and rheumatoid arthritis.

4. Altern Ther Health Med. 2010 Mar-Apr;16(2):32-40. Antioxidants and antiinflammatory dietary supplements for osteoarthritis and rheumatoid arthritis. Rosenbaum CC(1), O'Mathúna DP, Chavez M, Shields K. Author information: (1)Bethesda North Hospital Pharmacy, Cincinnati, Ohio, USA. drcathy@rxintegrativesolutions.com OBJECTIVE: To review efficacy studies of antioxidant and antiinflammatory dietary supplements used to manage osteoarthritis (OA) and rheumatoid arthritis (RA) and make conclusions about their place in therapy. Glucosamine, chondroitin, and methyl sulfonyl methane were excluded. DATA SOURCES: A literature search was conducted using MEDLINE (1996 through January 2009), EMBASE, Cochrane Library, Natural Medicines Comprehensive Database, and Natural Standard, with bibliographic review of relevant articles. Cited studies from before our search range were included if they represented the only published human data available. Search words included "antioxidant," "antiinflammatory," "cat's claw," "ginger," "fish oil," "omega-3," "turmeric," "vitamin E," "vitamin C," "Baikal skullcap," "barberry," "Chinese goldthread," "green tea," "Indian holy basil," "hu zhang,""oregano," and"rosemary." STUDY SELECTION AND DATA EXTRACTION: Efficacy studies published in English were included provided they evaluated the dietary supplements in patients with OA or RA. DATA SYNTHESIS: Our search strategy yielded 16 clinical studies (11 randomized, placebo-controlled clinical trials, three crossover trials, one case-controlled study, and one open-label study) in addition to one meta-analysis and one review article. CONCLUSIONS: Three studies support cat's claw alone or in combination for OA, and two studies support omega-3 fatty acids for the treatment of RA. We cannot recommend use of vitamin E alone; vitamins A, C, and E in combination; ginger; turmeric; or Zyflamend (New Chapter, Brattleboro, Vermont) for the treatment of OA or RA or omega-3 fatty acids for OA. Whether any of these supplements can be effectively and safely recommended to reduce nonsteroidal antiinflammatory drug or steroid usage is unclear and requires more high-quality research. PMID: 20232616 [Indexed for MEDLINE]

Benefits of basil tea for patients with differentiated thyroid cancer during radioiodine therapy: A randomized controlled trial.

5. Heliyon. 2023 Oct 5;9(10):e20691. doi: 10.1016/j.heliyon.2023.e20691. eCollection 2023 Oct. Benefits of basil tea for patients with differentiated thyroid cancer during radioiodine therapy: A randomized controlled trial. Nomura K(1), Nakayama M(1), Okizaki A(1). Author information: (1)Department of Radiology, Asahikawa Medical University, Hokkaido, Japan. BACKGROUND: Acute sialadenitis is one of the major physical complications of radioactive iodine therapy (RAIT) for differentiated thyroid cancer (DTC). It is considered necessary to pay attention to the psychological impact on the patient as well as the physical influence during RAIT. OBJECTIVE: To find evidence of the benefits of Basil tea on the psychological and physical side effects of RAIT. METHODS: Forty-four DTC patients after total thyroidectomy were randomly divided into Group A (Basil tea group, n = 22) and Group B (Control group, n = 22). Subjects in Group A drank 180 mL of Basil tea prepared from 2.0 g of Holy basil (Ocimum tenuiflorum Linn.) leaves after each meal for four days, starting on the day RAIT was performed. Those in Group B drank the same amount of distilled water after each meal for the same period as those in Group A. The State-Trait Anxiety Inventory (STAI) was used to assess anxiety, while the saliva component test, and salivary gland scintigraphy were used to assess the oral cavity. RESULTS: The rate of change of the STAI score (both State Anxiety and Trait Anxiety) was significantly lower in Group A than in Group B (P < 0.05). The rates of change of cariogenic bacteria, ammonia, protein, and occult blood were significantly lower in Group A than in Group B (P < 0.05). The rate of change of the washout ratio for salivary gland scintigraphy was significantly lower in Group B than in Group A (P < 0.05). CONCLUSIONS: Basil tea consumption not only protected against oral mucosal conditions and salivary gland disorders but also significantly relieved the patient's RAIT-related anxiety. Therefore, it was suggested that this tea could be useful for the maintenance of patients' QOL during RAIT. © 2023 The Authors. DOI: 10.1016/j.heliyon.2023.e20691 PMCID: PMC10565770 PMID: 37829808 Conflict of interest statement: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The authors received funding from Yamazaki Spice Promotion Foundation for this study.

An in vivo comparative clinical and radiographic evaluation of a mixture of zinc oxide-Ocimum sanctum extract, zinc oxide-ozonated oil, and zinc oxide-eugenol as obturating materials in primary molars.

6. J Indian Soc Pedod Prev Dent. 2022 Oct-Dec;40(4):398-403. doi: 10.4103/jisppd.jisppd_204_22. An in vivo comparative clinical and radiographic evaluation of a mixture of zinc oxide-Ocimum sanctum extract, zinc oxide-ozonated oil, and zinc oxide-eugenol as obturating materials in primary molars. Vachhani K(1), Rao D(1), Panwar S(1), Gupta S(1). Author information: (1)Department of Paediatric Dentistry, Pacific Dental College and Hospital, Udaipur, Rajasthan, India. BACKGROUND: To overcome some of the disadvantages of the current primary root canal obturating materials, there is a continued interest in search for chemical compounds with broader and more effective antibacterial action and less cytotoxicity. AIM: This study aimed to evaluate and compare in vivo the clinical and radiographic success of mixtures of zinc oxide-Ocimum sanctum extract, zinc oxide-ozonated oil, and zinc oxide-eugenol (ZOE) as obturating materials in pulpectomy of primary molars. SETTINGS AND DESIGN: This was an in vivo randomized controlled clinical trial. MATERIALS AND METHODS: Ninety primary molars selected were randomly divided into three groups. Group A was obturated with zinc oxide-O. sanctum extract, Group B with zinc oxide-ozonated oil, and Group C with ZOE. All the groups were evaluated for success or failure based on clinical and radiographic criteria at the end of 1, 6, and 12 months. STATISTICAL ANALYSIS USED: The intra- and inter-examiner reliability of the first and the second co-investigators was calculated by Cohen's kappa statistic. The data were analyzed using Chi-square test, P ≤ 0.05 (indicates statistical significance). RESULTS: By the end of 12 months, the overall clinical success rate was 88%, 95.7%, and 90.9% in Groups A, B, and C, respectively; whereas the radiographic success rate was found to be 80%, 91.3%, and 86.4% in Groups A, B, and C, respectively. CONCLUSION: On the basis of the overall success rates of all the three obturating materials, the following order of performance can be concluded: zinc oxide-ozonated oil > ZOE > zinc oxide-O. sanctum extract. DOI: 10.4103/jisppd.jisppd_204_22 PMID: 36861556 [Indexed for MEDLINE]

Modulation of growth performance, gut morphometry, and cecal microbiota in broilers by clove (Syzygium aromaticum) and tulsi (Ocimum sanctum) supplementation.

7. Poult Sci. 2023 Jan;102(1):102266. doi: 10.1016/j.psj.2022.102266. Epub 2022 Oct 20. Modulation of growth performance, gut morphometry, and cecal microbiota in broilers by clove (Syzygium aromaticum) and tulsi (Ocimum sanctum) supplementation. Islam R(1), Sultana N(2), Bhakta S(1), Haque Z(1), Hasan A(3), Siddique MP(3), Islam MR(1). Author information: (1)Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. (2)Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. Electronic address: nasrin.sultana@bau.edu.bd. (3)Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. In an epoch of the growing risk of antibiotic resistance, there is a dire need to establish an effective novel feeding practice for broiler nutrition as an alternative to antibiotics. Hence, the aim of the current study was to evaluate the impact of clove powder and tulsi extract on the growth performance, gut morphologic and morphometric indices, and cecal microbial status of broiler, as an alternative to antibiotic growth promoters (AGPs). Sixty day-old chicks of Cobb-500 strain were randomly divided into 4 groups, each having 15 birds. Chicks of the control group (T0) were fed commercial broiler feed with no additional supplementation. The treatment groups were offered commercial broiler feed and received clove powder and tulsi extract with drinking water at the rate of 0.5% + 2% (T1), 1% + 3% (T2), and 1.5% + 4% (T3), respectively. Results showed a nonlinear relationship with the dosage of clove and tulsi. All the growth parameters substantially (P < 0.05) improved in T2 while T1 and T3 showed no significant improvement compared to T0. The final body weight was significantly (P < 0.05) higher in T2. Giblet and offal weights showed no noticeable differences except in the intestine and heart where intestine weight markedly (P < 0.05) decreased in T3 and heart weight significantly (P < 0.05) increased in T1 and T2. Clove and tulsi supplementation substantially improved the villus height and villus surface area of the small intestine in T2 while the large intestine remained mostly unaffected by the treatment. Cecal microbial status significantly improved in all the treatment groups having increased (P < 0.05) Lactobacillus spp. count and decreased (P < 0.05) E. coli count compared to T0. Based on the aforementioned findings, it can be concluded that the combination of clove and tulsi can improve the growth performance and gut health of broilers which is largely dose-dependent and might be supplied as a potential alternative to AGPs. Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved. DOI: 10.1016/j.psj.2022.102266 PMCID: PMC9660731 PMID: 36370662 [Indexed for MEDLINE]

Antibacterial efficacy of manuka honey, ocimum sanctum, curcuma longa and 0.2% chlorhexidine mouthwash on the level of streptococcus mutans and lactobacillus acidophilus - A randomized controlled trial.

8. Indian J Dent Res. 2022 Apr-Jun;33(2):169-173. doi: 10.4103/ijdr.ijdr_159_22. Antibacterial efficacy of manuka honey, ocimum sanctum, curcuma longa and 0.2% chlorhexidine mouthwash on the level of streptococcus mutans and lactobacillus acidophilus - A randomized controlled trial. Jain A(1), Singh V(1), Lukram A(2), Chatterjee S(3), Khan AM(4), Dawar G(5). Author information: (1)Department of Public Health Dentistry, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, Uttar Pradesh, India. (2)Dental College, JNIMS, Manipur, India. (3)Department of Public Health Dentistry, Hazaribag, Jharkhand, India. (4)Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Moradabad, India. (5)Department of Oral Pathology and Microbiology, Teerthanker Mahaveer Dental College and Research Centre, Moradabad, India. OBJECTIVE: : To assess the effects of Manuka honey, Ocimum sanctum, Curcuma longa, and 0.2% chlorhexidine mouthwash on Streptococcus mutans and Lactobacillus acidophilus levels. MATERIAL AND METHODS: A randomized controlled trial will be conducted on dental students of Teerthanker Mahaveer Dental College and Research Centre, Moradabad. The study participants will be divided into four groups. Each group will have a total of 20 individuals. By using a lottery system, Group A (Manuka honey mouthrinse), Group B (Ocimum sanctum mouthrinse), Group C (Curcuma longa mouthrinse), and Group D (0.2% chlorhexidine mouthrinse) will be chosen. To match the circadian cycle, saliva will be collected at baseline and again after 2 weeks between 10 and 10.30 a.m. The sterile container will subsequently be delivered to the microbiological laboratory and processed as soon as possible to measure Streptococcus mutans and Lactobacillus acidophilus count. For 2 weeks, participants were told to use 10 mL of mouthrinse twice daily. RESULTS: : The mean oral hygiene index-simplified (OHI-S) score of all the four groups showed reduction in their scores from baseline to after the study period. For both S. mutans and L. acidophilus, there was a substantial Percentage Reduction (PR) between the prerinse and postrinse samples in all four groups. DISCUSSION: : Because quantitative actions play a crucial part in the caries disease process, the changes in microbial activity before and after administration of experimental mouthwashes were examined. CONCLUSION: : Essential oil aqueous extracts were as efficient antibacterial mouthwashes as chlorhexidine and iodine mouthwashes. DOI: 10.4103/ijdr.ijdr_159_22 PMID: 36254954 [Indexed for MEDLINE] Conflict of interest statement: None

Randomized placebo-controlled pilot clinical trial on the efficacy of ayurvedic treatment regime on COVID-19 positive patients.

9. Phytomedicine. 2021 Apr;84:153494. doi: 10.1016/j.phymed.2021.153494. Epub 2021 Feb 4. Randomized placebo-controlled pilot clinical trial on the efficacy of ayurvedic treatment regime on COVID-19 positive patients. Devpura G(1), Tomar BS(2), Nathiya D(3), Sharma A(1), Bhandari D(4), Haldar S(5), Balkrishna A(6), Varshney A(7). Author information: (1)Department of Medicine, National Institute of Medical Sciences, Jaipur-Delhi Highway (NH-11C), Jaipur 303121, Rajasthan, India. (2)Department of Pediatrics, National Institute of Medical Sciences, Jaipur-Delhi Highway (NH-11C) Expy, Shobha Nagar, Jaipur 303121, Rajasthan, India. Electronic address: chancellor@nimsuniversity.com. (3)Department of Clinical Pharmacology, National Institute of Medical Sciences, Jaipur-Delhi Highway (NH-11C), Jaipur 303121, Rajasthan, India. (4)Patanjali Chikitsalya, Amrapali Circle, Vaishali Nagar, Jaipur 302021, Rajasthan, India. (5)Drug Discovery and Development Division, Patanjali Research Institute, Roorkee-Haridwar Road, Haridwar 249405, Uttarakhand, India. (6)Drug Discovery and Development Division, Patanjali Research Institute, Roorkee-Haridwar Road, Haridwar 249405, Uttarakhand, India; Department of Allied and Applied Sciences, University of Patanjali, NH-58, Haridwar 249405, Uttarakhand, India. (7)Drug Discovery and Development Division, Patanjali Research Institute, Roorkee-Haridwar Road, Haridwar 249405, Uttarakhand, India; Department of Allied and Applied Sciences, University of Patanjali, NH-58, Haridwar 249405, Uttarakhand, India. Electronic address: anurag@prft.co.in. BACKGROUND: Specific treatment for COVID-19 is still an unmet need. Outcomes of clinical trials on repurposed drugs have not been yielding success. Therefore, it is necessary to include complementary approaches of medicine against COVID-19. PURPOSE: This study was designed to evaluate the impact of traditional Indian Ayurvedic treatment regime on asymptomatic patients with COVID-19 infection. STUDY DESIGN: It is a placebo controlled randomized double-blind pilot clinical trial. METHODS: The study was registered with Clinical Trial Registry-India (vide Registration No. CTRI/2020/05/025273) and conducted at the Department of Medicine in National Institute of Medical Sciences and Research, Jaipur, India. 1 g of Giloy Ghanvati (Tinospora cordifolia) and 2 g of Swasari Ras (traditional herbo-mineral formulation) and 0.5 g each of Ashwagandha (Withania somnifera) and Tulsi Ghanvati (Ocimum sanctum) were given orally to the patients in treatment group twice per day for 7 days. Medicines were given in the form of tablets and each tablet weighed 500 mg. While, Swasari Ras was administered in powdered form, 30 min before breakfasts and dinners, rest were scheduled for 30 min post-meals. Patients in the treatment group also received 4 drops of Anu taila (traditional nasal drop) in each nostril every day 1 h before breakfast. Patients in the placebo group received identical-looking tablets and drops, post randomization and double blinded assortments. RT-qPCR test was used for the detection of viral load in the nasopharyngeal and oropharyngeal swab samples of study participants during the study. Chemiluminescent immunometric assay was used to quantify serum levels of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and high sensitivity C-reactive protein (hs-CRP) on day 1 and day 7 of the study. RESULTS: By day 3, 71.1 % and 50.0 % patients recovered in the treatment and placebo groups, respectively. Treatment group witnessed 100 % recovery by day 7, while it was 60.0 % in the placebo group. Average fold changes in serum levels of hs-CRP, IL-6 and TNF-α in treatment group were respectively, 12.4, 2.5 and 20 times lesser than those in the placebo group at day 7. There was 40 % absolute reduction in the risk of delayed recovery from infection in the treatment group. CONCLUSIONS: Ayurvedic treatment can expedite virological clearance, help in faster recovery and concomitantly reduce the risk of viral dissemination. Reduced inflammation markers suggested less severity of SARS-CoV-2 infection in the treatment group. Moreover, there was no adverse effect observed to be associated with this treatment. Copyright © 2021. Published by Elsevier GmbH. DOI: 10.1016/j.phymed.2021.153494 PMCID: PMC7857981 PMID: 33596494 [Indexed for MEDLINE] Conflict of interest statement: Authors declare no conflict of interests with regards to the submitted work. The medications were provided by Divya Pharmacy, Haridwar, Uttarakhand, India. Acharya Balkrishna is an honorary trustee in Divya Yog Mandir Trust. Besides, providing the medications, Divya Pharmacy was not involved in any aspect of the clinical trial reported in this study. Clinical trial was conducted at National Institute of Medical Sciences, Jaipur, India.

Efficacy of Improvised Topical Zinc (1%) Ora-Base on Oral Mucositis during Cancer Chemo-Radiation-A Randomized Study.

10. J Nutr Sci Vitaminol (Tokyo). 2020;66(2):93-97. doi: 10.3177/jnsv.66.93. Efficacy of Improvised Topical Zinc (1%) Ora-Base on Oral Mucositis during Cancer Chemo-Radiation-A Randomized Study. Chaitanya N(1), Badam R(1), Aryasri AS(2), Pallarla S(1), Garlpati K(3), Akhila M(3), Soni P(3), Gali S(1), Inamdar P(4), Parinita B(1), Zaheer K(3), Prabhath T(1), Swetha A(1). Author information: (1)Department of Oral Medicine and Radiology, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre. (2)Department of Orthodontics and Dentofacial Orthopedics, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre. (3)Department of Oral Medicine, Panineeya Mahavidyalaya Institute of Dental Sciences and Research Centre. (4)Department of Oral Diagnostics, Rutgers School of Dental Medicine. Oral mucositis refers to erythematous and ulcerative lesions of oral mucosa during chemo/radiotherapy. Treatment modalities were directed towards reduction in severity of oral mucositis. Zinc plays an important role to retard oxidative processes and is considered as the critical component in wound healing. To compare the efficacy of zinc alone with improvised zinc preparation in reducing the severity of oral mucositis. Improvised zinc was a combination of zinc oxide, amla, tulsi and curcumin at 1% therapeutic concentrations. Seventy-five subjects undergoing chemo/radiotherapy were randomly divided into three groups: Group A (25 subjects) had received topical 5% zinc oxide paste trice daily application after food for entire treatment period, initiated 2 d prior to radiotherapy. Group B (25) received improvised zinc preparations (1%) and instructed to apply same as group A. Group C (25) received standard treatment offered by cancer hospital. All the groups were assessed for oral mucositis using WHO grading system at 7th, 14th, 21st, 28th, 35th day by the oncologist and results were tabulated for statistical analysis. Severity of oral mucositis reduced in zinc and improvised zinc group (p=0.096) when compared with controls with significant p value (0.037). Comparison of improvised zinc preparation (1%) group with only zinc group revealed that severity of overall mucositis though was not significant, was less in improvised zinc group with p value (0.029, 0.013) at 28 and 35 d respectively. Improvised zinc administration during radiation therapy was beneficial in reduction of oral mucositis during cancer treatment. DOI: 10.3177/jnsv.66.93 PMID: 32350185 [Indexed for MEDLINE]

A comparative evaluation of the anticaries efficacy of herbal extracts (Tulsi and Black myrobalans) and sodium fluoride as mouthrinses in children: A randomized controlled trial.

11. Indian J Dent Res. 2018 Nov-Dec;29(6):760-767. doi: 10.4103/ijdr.IJDR_790_16. A comparative evaluation of the anticaries efficacy of herbal extracts (Tulsi and Black myrobalans) and sodium fluoride as mouthrinses in children: A randomized controlled trial. Megalaa N(1), Thirumurugan K(2), Kayalvizhi G(3), Sajeev R(3), Kayalvizhi EB(4), Ramesh V(3), Vargeese A(3). Author information: (1)Department of Pedodontics and Preventive Dentistry, Sri Venkateshwaraa Dental College, Puducherry, India. (2)Department of Oral and Maxillofacial Surgeon, Mahatma Gandhi Post Graduate Institute of Dental Sciences, Puducherry, India. (3)Department of Pedodontics and Preventive Dentistry, Indira Gandhi Institute of Dental Sciences, Puducherry, India. (4)Department of Oral Medicine and Radiology, Madha Dental College and Hospital, Chennai, India. BACKGROUND: Dental caries is a multifactorial disease in which microorganisms play an important role. Recently, herbs have been tried as mouthrinses to combat the side effects of chemical mouthrinses. The anticaries efficacy of Sodium fluoride, Tulsi leaf, and Black myrobalans fruit extracts on Streptococcus mutans (S. mutans) have been reported in the literature, but no comparative study has been done yet. AIM: This study aims to observe the change in the pH of saliva and to assess the efficacy of the herbal rinses-Tulsi and Black myrobalans on S. mutans count while comparing it with Sodium fluoride mouthrinse. METHODS: Herbal ethanolic extracts of Tulsi (4%) and Black myrobalans (2.5%) were prepared as mouthrinses and compared with sodium fluoride mouthrinse (0.05%). Sixty high caries risk patients were selected and allocated randomly into three groups [n = 20], categorized as Group A-Sodium fluoride mouthrinse, Group B-Tulsi mouthrinse, and Group C-Black myrobalans mouthrinse. They were instructed to rinse their mouth with their assigned mouthrinses for 7 days. Salivary samples were collected and sent to the laboratory at baseline, 1 h postrinsing and after 7th day of rinsing for determining the salivary pH and S. mutans count. The increase in pH and reduction of S. mutans were determined. The values obtained were tabulated and statistically analyzed. RESULTS: There was a significant increase in the salivary pH and reduction in S. mutans count after rinsing in all the three groups. Increase in salivary pH was more in the Sodium fluoride mouthrinse when compared to the experimental herbal groups (Group B and Group C). While S. mutans counts reduced more with Tulsi mouthrinse at 1 h postrinsing and after the 7th day of rinsing more reduction was seen in Black myrobalans mouthrinse group. CONCLUSION: The results of the study suggest that herbal mouthrinses could be tried as an adjunctive anticaries agent against dental caries causing microorganisms. DOI: 10.4103/ijdr.IJDR_790_16 PMID: 30589005 [Indexed for MEDLINE] Conflict of interest statement: None

Potential of native Thai aromatic plant extracts in antiwrinkle body creams.

12. J Cosmet Sci. 2015 Jul-Aug;66(4):219-31. Potential of native Thai aromatic plant extracts in antiwrinkle body creams. Leelapornpisid P, Wickett RR, Chansakaow S, Wongwattananukul N. Antioxidant activities of 10 essential oils and 10 absolutes extracted from Thai aromatic plants were evaluated and compared to thyme oil, trolox, quercetin, and kaempferol by two independent assays: the 2, 2-diphenyl-1-1-picrylhydrazyl (DPPH*) radical scavenging assay and the thiobarbituric acid reactive species (TBARS) assay for lipid peroxidation. We found that four essential oils including ginger oil (Zingiber officinale Roscoe), Wan-sao-long leaf oil (Amomum uliginosum Koen), lemongrass oil (Cymbopogon citratus), holy basil oil (Ocimum sanctum L.), and the absolute of dwarf ylang-ylang [Cananga odorata Hook. f. & Thomson var. fruticosa (Craib) J. Sinclair] exhibited high antioxidant activity in both DPPH and TBARS assays and possessed satisfactory fragrance properties. These were then combined into an essential oil blend (EOB) and retested for antioxidant activity. The EOB also exhibited high antioxidant activity in the above assays. It was then incorporated into a stable cream base as EOB body cream. The EOB body cream was found to be best able under storage in stress conditions and presented significantly higher antioxidant activity than its' cream base both before and after stability testing. The effect of EOB body cream on skin surface topography was evaluated in 29 healthy volunteers using the Skin Visiometer (SV 600 FW, CK Electronic GmbH, Germany). Three parameters, Ra, Rz (roughness), and surface, were analyzed. After 4 weeks of application, the EOB body cream showed significant reductions in surface and Rz compared with before treatment (p < 0.05, paired t-test), and with untreated and placebo treatment (p < 0.05, Duncan test). These results indicate that the essential oils and absolutes from Thai plants may serve as potential sources of natural antioxidants for spa and cosmetic products designed to prevent or treat signs of skin aging. PMID: 26665978 [Indexed for MEDLINE]

Holy basil (Ocimum sanctum Linn.) leaf extract enhances specific cognitive parameters in healthy adult volunteers: A placebo controlled study.

13. Indian J Physiol Pharmacol. 2015 Jan-Mar;59(1):69-77. Holy basil (Ocimum sanctum Linn.) leaf extract enhances specific cognitive parameters in healthy adult volunteers: A placebo controlled study. Sampath S, Mahapatra SC, Padhi MM, Sharma R, Talwar A. INTRODUCTION: Ocimum sanctum (OS), known as Holy basil, has been documented to possess neuroprotective, cognition-enhancing and stress relieving effects in animal models. However there is paucity of clinical studies to document these effects. MATERIALS AND METHODS: Effect of OS on parameters related to cognition and stress in humans was evaluated with administration of 300 milligram capsules of ethanolic leaf extracts of Ocimum sanctum (EtOS) or placebo per day, over 30 days. RESULTS: Intra-group comparison of Sternberg and Stroop test showed improvement in both the placebo and EtOS groups, however, the improvement stabilized after day 15 in the placebo group. Intergroup comparison revealed a significant improvement of the following cognitive parameters in the EtOS as compared to the placebo: reaction time (RT) and error rate (ER) of Sternberg test, RT of neutral task of Stroop, RT and ER of interference task of Stroop. The intra-group comparison of P300 latency, salivary cortisol, and State-Trait Anxiety Inventory showed improvement over time in the EtOS group alone, though the inter-group difference was significant in the P300 latency alone. There were no changes in heart rate (HR), AHR, or galvanic skin response (GSR) or AGSR. CONCLUSION: Ocimum sanctum leaf extract seems to have potential cognition-enhancing properties in humans. PMID: 26571987 [Indexed for MEDLINE]

Ethnobotanical survey of malaria prophylactic remedies in Odisha, India.

14. J Ethnopharmacol. 2013 Apr 19;146(3):768-72. doi: 10.1016/j.jep.2013.02.003. Epub 2013 Feb 19. Ethnobotanical survey of malaria prophylactic remedies in Odisha, India. Nagendrappa PB(1), Naik MP, Payyappallimana U. Author information: (1)Institute of Ayurveda and Integrative Medicine (I-AIM), (Formerly Foundation for Revitalisation of Local Health Traditions, FRLHT) No. 74/2, Jarakabande kaval, Attur P.O., Via Yelahanka, Bangalore-560 106, Karnataka, India. bn.prakash@frlht.org AIM OF THE STUDY: To investigate the plants traditionally used for prevention of malaria in Cuttack, Gajapati and Koraput districts of Odisha state, eastern India. MATERIALS AND METHODS: An ethnobotanical survey was carried out among 20 traditional healers who were sampled based on recommendations of local elders and local non-government organizations. Data were collected through semi-structured interview. RESULTS: The study revealed the use of 16 traditional plant species belonging to 12 families for prevention of malaria. Andrographis paniculata, Azadirachta indica, Nyctanthes arbor-tristis, Ocimum sanctum, Piper nigrum, Zingiber officinale were the most commonly reported plants for their malaria prophylactic use by the healers of three districts of Odisha. Most of the remedies were used in decoction form. CONCLUSIONS: Findings of this study provide a lead to explore traditional plants for malaria preventive potential through further pre-clinical and clinical studies. Copyright © 2013 Elsevier Ireland Ltd. All rights reserved. DOI: 10.1016/j.jep.2013.02.003 PMID: 23434608 [Indexed for MEDLINE]

Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum Linn.) leaf extract on healthy volunteers.

15. J Ethnopharmacol. 2011 Jul 14;136(3):452-6. doi: 10.1016/j.jep.2011.05.012. Epub 2011 May 17. Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum Linn.) leaf extract on healthy volunteers. Mondal S(1), Varma S, Bamola VD, Naik SN, Mirdha BR, Padhi MM, Mehta N, Mahapatra SC. Author information: (1)Department of Physiology, All India Institute of Medical Sciences, New Delhi, India. ETHNOPHARMACOLOGICAL RELEVANCE: Tulsi (Ocimum sanctum Linn.) is considered as a sacred herb and traditionally it is believed that consumption of Tulsi leaf on empty stomach increases immunity. Experimental studies have shown that alcoholic extract of Tulsi modulates immunity. MATERIALS AND METHODS: The present study was designed to evaluate the immunomodulatory effects of ethanolic extract of Tulsi leaves through a double-blinded randomized controlled cross-over trial on healthy volunteers. Three hundred milligrams capsules of ethanolic extracts of leaves of Tulsi or placebo were administered to 24 healthy volunteers on empty stomach and the results of 22 subjects who completed the study were analyzed. The primary objective was to study the levels of Th1 and Th2 cytokines (interferon-γ and interleukin-4) during both pre and post intervention period in blood culture supernatants following stimulation with lipopolysaccharide and phytohaemagglutinin. Other immunological parameters such as T-helper and T-cytotoxic cells, B-cells and NK-cells also were analyzed using Flowcytometry. RESULTS: Statistically significant increase in the levels of IFN-γ (p=0.039), IL-4 (p=0.001) and percentages of T-helper cells (p=0.001) and NK-cells (p=0.017) were observed after 4 weeks in the Tulsi extract intervention group in contrast to the placebo group. CONCLUSIONS: These observations clearly ascertain the immunomodulatory role of Tulsi leaves extract on healthy volunteers. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. DOI: 10.1016/j.jep.2011.05.012 PMID: 21619917 [Indexed for MEDLINE]

In vivo enhancement of natural killer cell activity through tea fortified with Ayurvedic herbs.

16. Phytother Res. 2010 Jan;24(1):129-35. doi: 10.1002/ptr.2889. In vivo enhancement of natural killer cell activity through tea fortified with Ayurvedic herbs. Bhat J(1), Damle A, Vaishnav PP, Albers R, Joshi M, Banerjee G. Author information: (1)Unilever Food and Health Research Institute, Unilever Research India, 64, Main Road, Whitefield, Bangalore 560066, India. The effect of a tea fortified with five herbs selected from Indian traditional medicine (Ayurveda) for their putative immunoenhancing effect (Withania somnifera, Glycyrrhzia glabra, Zingiber officinale, Ocimum sanctum and Elettaria cardamomum) on innate immunity was investigated. Ex vivo natural killer (NK) cell activity was assessed after consumption of fortified tea compared with regular tea in two independent double-blind intervention studies. Both studies were conducted in India with healthy volunteers (age >or= 55 years) selected for a relatively low baseline NK cell activity and a history of recurrent coughs and colds. In a pilot study conducted with 32 volunteers, the consumption of Natural Care tea significantly improved the NK cell activity of the volunteers in comparison with a population consuming regular tea. These results were validated in an independent crossover study with 110 volunteers. Data from these two studies indicate that regular consumption of the tea fortified with Ayurvedic herbs enhanced NK cell activity, which is an important aspect of the (early) innate immune response to infections. (c) 2009 John Wiley & Sons, Ltd. DOI: 10.1002/ptr.2889 PMID: 19504465 [Indexed for MEDLINE]